MicroVal has approved the issuance of the certificate for the SureFast® Listeria 3plex ONE Kit and the SureFast® PREP Bacteria Kit, developed by Congen Biotechnologie GmbH. The study is being conducted by Q Laboratories.
The SureFast® Listeria 3plex ONE Kit enables real-time PCR detection of Listeria species and Listeria monocytogenes in food and environmental samples. The kit can also be used in combination with the SureFast® PREP Bacteria Kit, offering an alternative DNA extraction option. The method consists of four steps: enrichment, DNA extraction, real-time PCR detection, and interpretation of results. The real-time PCR assay can be performed using commercially available PCR instruments equipped to detect four fluorescence channels OX, Cy5, VIC, and HEX simultaneously.
The validation has been conducted in accordance with ISO 16140-2:2016 and the reference methods ISO 11290-1:2017 and ISO 6887 (Parts 1–5). The study covers the detection of Listeria spp. and Listeria monocytogenes in a broad range of foods and environmental samples, including:
- Heat-processed and raw milk and dairy products
- Raw and ready-to-eat (RTE) meat products
- RTE poultry products
- RTE and ready-to-reheat (RTRH) fishery products
- Fresh produce and fruits
- Multi-component foods or meal components
- Environmental samples
This validation confirms the performance of the SureFast® Listeria 3plex ONE Kit and SureFast® PREP Bacteria Kit as reliable alternatives for the detection of Listeria spp. and Listeria monocytogenes across diverse food and environmental matrices.
The certificate and detailed summary report (number 2023LR114) are available on our website.