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MicroVal Renews Certification for Hygiena’s foodproof® Salmonella Detection Kit

MicroVal has renewed the certification for Hygiena Diagnostics GmbH’s foodproof® Salmonella Detection Kit (Cat. No. R 310 27 or R 302 27), combined with Reagent D (Cat. No. A 500 02) and foodproof® StarPrep One Kit (Cat. No. S 400 07) or foodproof® Magnetic Preparation Kit IV (Cat. No. S 400 15) for DNA extraction. It is designed for reliable detection of Salmonella spp. and is intended to be used in combination with the foodproof ® Enterobacteriaceae plus Cronobacter Detection Kit (Cat. No. R 310 15.1 or R 302 15.1) for infant formulae and related matrices. Both kits were validated in a joint study.

Initially validated in 2012, the kit meets the rigorous standards set by EN ISO 16140-2:2016, with reference method ISO 6579-1:2017. It is certified for use in testing infant formula and infant cereals, probiotics containing products, ingredients, and environmental samples.

The certificate and summary report are available on the MicroVal website under study number 2011LR39.

Extension certificates Bentley Instruments BactoCount IBC 3.0 & BactoCount IBCm 3.0 issued

MicroVal certified an extension study for the Bentley Instruments BactoCount IBC 3.0 & BactoCount IBCm 3.0 devices. These devices measure total bacterial count (TBC) and somatic cell count (SCC). Initially validated in 2022 for raw cow’s milk, they are now certified for raw goat’s and sheep’s milk as well. Both validation studies were conducted by ACTALIA Cecalait.

The BactoCount IBC 3.0 & BactoCount IBCm 3.0 can offer simultaneous real-time analysis of TBC and SCC in raw milk with a flow cytometer, but in the frame of this validation, TBC and SCC were tested separately. The validation scope covers only the separate use of TBC and SCC.

The extension studies were done according to ISO 16297, ISO 21187, and ISO 16140-2 for TBC and according to ISO 8196-3, ISO 13366-2, and ISO 16140-2 for SCC, using ISO 4833-1 and ISO 133366-1 as reference methods. Results obtained for TBC were also compared with the limits defined in the EU-RL document. The programs used to analyze sheep’s and goat’s milk are the same as for cow milk (ratio milk/reagent, type of reagent, incubation time, and protocol).

Both certificates 2021LR97 and 2021LR98 can be found on our website.

Certification for Neogen’s Soleris Non-Fermenting Total Viable Count (NF-TVC)

In September, MicroVal granted certification for Non-Fermenting Total Viable Count (NF-TVC). NF-TVC is a series of Soleris manufactured by Neogen. NF-TVC detects total viable count at a threshold of 10 cfu per gram across a wide variety of food products.

The NF-TVC vial, containing 9 mL of broth, is designed for dilute-to-specification monitoring in diverse food matrices. As microorganisms grow in the medium, the carbon dioxide produced diffuses through a membrane into a soft agar plug with a dye indicator, resulting in a color change that is analyzed by the Soleris® instrument.

The detection time correlates with the contamination level, allowing for quicker results with higher levels of contamination. This validation aims to establish a rapid screening method for increased throughput in monitoring various food products, thus reducing both analysis time and time to results.

Campden BRI conducted the validation study in accordance with ISO 16140-2:2016, using ISO 4833-1:2013 as a reference method. The study encompasses a broad range of food categories, including dairy, fishery products (raw and ready-to-cook), fresh and processed produce, multicomponent foods, and meat and poultry products (raw and ready-to-cook).

The certificate and the detailed summary report, number 2022LR113, are available on our website.

MicroVal welcomes a new MicroVal Technical Committee Member

Last July, MicroVal announced the addition of three new members to its Technical Committee (MVTC). This month, MicroVal is pleased to welcome another new member: Stephen Antonie-Zijlstra from FrieslandCampina. With his appointment, the MVTC now comprises 29 members.

As an MVTC member, candidates are expected to have expertise in microbiological reference and alternative methods, an area where Stephen excels. He brings extensive experience in scouting, validating, and verifying various microbiological methods, as well as in assessing their performance in routine laboratory work. Additionally, he has been involved in the validation and verification of alternative methods prior to their implementation in his lab. Stephen is also an active participant in several ISO working groups and International Dairy Federation (IDF) Action Teams. The current MVTC members believe he will contribute valuable insights, particularly in the ISO 16140-2 validation studies of alternative microbiological methods.

The MVTC consists of Campden BRI (UK), Merck Life Science / MilliporeSigma (USA), Danone (France), ADRIA (France), Nestlé (Switzerland), Mérieux NutriSciences – Global (USA), Hygiena (France), bioMérieux (Spain), Q Laboratories (USA), TLR International Laboratories (The Netherlands), Laboratoire Microsept (France), Neogen Corporation (France), FDA (USA), Thermo Fisher Scientific (UK), Eurofins WFC (The Netherlands), AOAC INTERNATIONAL consultant (USA), NVWA (The Netherlands), LRQA (The Netherlands), Actalia (France), Bio-Rad Laboratories (France), IEH Laboratories and Consulting Group (USA), and FrieslandCampina (The Netherlands).

Contact details can be found on this page on our website.

Flashback MicroVal session at the AOAC International

MicroVal reflects on a successful participation at the AOAC International conference 2024 in Baltimore.

The session began with Joost Witsenburg introducing MicroVal, followed by Kate Mastovska (AOAC) who highlighted the collaboration between AOAC and MicroVal. Douglas Marshall (Eurofins), Frederic Martinez (Neogen), and Daniele Sohier (Hygiena) then led an engaging discussion on method validation using larger test portion sizes. Lastly, Katherine Evans (Thermo Fisher Scientific), Tom Hammack (IEH), Sophie Pierre (Bio-Rad), and Erin Crowley (Q Laboratories) shared their success stories in validation and certification within the MicroVal framework.

Microval welcomes three new MicroVal Technical Committee Members

MicroVal (NEN) is happy to welcome three new members of the MicroVal Technical Committee (MVTC). They are Eva van Hees (TLR International Laboratories), Guodong Zhang (FDA), and Kirsten van Kooten (Eurofins WFC). With them, MicroVal has 28 members for this technical committee.

The #MVTC governs the validation process towards #certification and conducts #validation studies. It consists of voluntary members with a relevant background in #microbiological #food #safety, #laboratory #research, #method validation, and certification. The MVTC shall strive to have a balanced representation of the different stakeholders so that no single interest predominates. Admission for new members to the MVTC will be judged on a case-by-case basis. They are appointed for a period of four years.

Contact details can be found on this page on our website.

Three One Plate certificates issued

MicroVal has approved issuing three certificates for One Plate by Neogen Cooperation. Campden BRI has carried out these validation studies of this alternative method based on ISO 16140-2:2016.

The first certificate is One Plate Yeast & Mould. The validation is for the enumeration of yeasts and moulds. This One Plate has been validated against ISO 21527-1:2008 and ISO 21527-2:2008. The scope of validation is a broad range of foods consisting of the following categories:

Heat-processed dairy products
Confectionary, bakery, and eggs (combined category)
Fresh produce and fruit
Ready-to-eat foods (combined category: meat, poultry, and fishery products)
Multicomponent foods or meal components

The second certificate is One Plate Enterobacteriaceae & E.coli. The validation is for the enumeration of Enterobacteriaceae. This second type of One Plate has been validated against ISO 21528-2:2018. Like the first study, the scope of the validation study is a broad range of foods, with categories included:

Milk and dairy products
Fresh produce and fruits
Raw poultry and meats (Combined category raw/ RTC meats and poultry)
Ready-to-eat foods (Combined category RTE/RTRH meats, poultry, and fish)
Multicomponent foods

The last certificate is One Plate Total Viable Count. One Plate for Total Viable Count (OP-TVC) offers a rapid method for enumerating total aerobic mesophiles using traditional culture methodology. It has been validated against ISO 4833-1:2013. Although the scope of the validation study is also a broad range of foods, the categories are:

Heat processed dairy
Raw fishery products
Fresh and heat-processed produce
Multicomponent products
Raw meat and poultry products

On our website, three certificates, 2021LR99, 2022LR108, and 2022LR112, can be found under ‘issued certificates / alternative methods for detection and enumeration‘.

MicroVal at the AOAC INTERNATIONAL 2024 Annual Meeting & Exposition

We are excited to announce that MicroVal will be hosting a session at the AOAC INTERNATIONAL 2024 Annual Meeting & Exposition in Baltimore, USA.

Curious about what MicroVal is and how we operate? Join us on Monday, August 26, 2024, from 17:30 to 18:30 in the Essex meeting room for an in-depth look at MicroVal. During this session, members of the MicroVal committees will present an overview of our activities, the collaboration between AOAC and MicroVal, the validation of larger test portion sizes, and share success stories.

Don’t miss this opportunity to learn more about MicroVal. Joost Witsenburg and Eric Kamst from the MicroVal secretariat, along with many others involved, will be present and eager to meet and engage with you.

Attendance of the MicroVal session is free. For information on how to register for the Annual Meeting, please visit the AOAC INTERNATIONAL website: 2024 Annual Meeting Registration – AOAC INTERNATIONAL

Mark your calendar and join us at the AOAC INTERNATIONAL 2024 Annual Meeting & Exposition in Baltimore! We look forward to seeing you there!

Microval welcomes two new MicroVal General Committee Members

MicroVal is happy to welcome two new members of the MicroVal General Committee (MGC). Yannick Bichot (Bio-Rad Laboratories) and Frédéric Martinez (Neogen Corporation). With them, there are currently 16 members in the MGC.

The MGC is responsible for setting up standardized procedures, complementary to ISO 16140-2 and ISO 16140-6, for conducting the validation studies and to make sure that those validation studies stay in line with current legislation. In addition, the MGC promotes MicroVal in order to attract new validation assignments. All MGC members participate on a voluntary basis originating from different relevant backgrounds like food safety, regulatory affairs and standardization, method validation, and certification.

Organizations represented in the MGC are of Nestle (Switzerland), Hygiena (Germany), bioMérieux (France), Eurofins (USA), Neogen Corporation (USA), Merck KGaA (Germany), Anses (France), ADRIA Developpement (France), Post Consumer Brands (USA), NVWA (The Netherlands), LRQA (The Netherlands), R-Biopharm AG (Germany), Bio-Rad Laboratories (France), and Liaison members of AOAC International (USA) and NordVal (Scandinavia and Baltics).

Contact details can be found on this page on our website.

First certificate for Compact Dry CFR

In May 2024, MicroVal approved the issuing of a certificate for Compact Dry CFR, manufactured by Shimadzu Diagnostics Corporation. Compact Dry CFR features ready-to-use dry media sheets comprising a culture medium and a cold-soluble gelling agent, rehydrated by inoculating 1 ml diluted sample into the center of the self-diffusible medium. This chromogenic plate is designed for enumerating Coliforms. After incubation at 35±1°C for 16-18h, Coliforms develop into blue/blue-green colonies due to chromogens in the medium.

Campden BRI conducted the validation study as the Microval Expert Laboratory, following the ISO 16140-2:2016. The scope of the validation study is two categories: raw milk and dairy products and heat-processed milk and dairy products. For this, the ISO 4832:2006 was used as a reference method. The certificate and the detailed summary report, number 2023LR123, are available on our website.