A MicroVal validation study conducted by ADRIA has successfully evaluated the NEOGEN® Molecular Detection Assay 2 for the detection of Shiga-toxin-producing Escherichia coli (STEC) and Shiga-toxin gene (stx1 and stx2) and intimin gene (eae) from Shiga-toxin-producing E. coli STEC. The study was performance in accordance with ISO 16140-2 & ISO 16140-2/A1:2024 with ADRIA internal method based on ISO/TS 13136 (November 2012) as reference method. The study covered raw meat (except poultry) as well as fresh produces and fruits.
The validation scope included two alternative methods:
- NEOGEN® Molecular Detection Assay 2 – STEC Gene Screen (stx)
- NEOGEN® Molecular Detection Assay 2 – STEC Gene Screen (stx and eae)
After standardised enrichment steps using pre-warmed Buffered Peptone Water (BPW), DNA extraction was performed with the NEOGEN Lysis Solution, followed by LAMP-based detection targeting stx alone or stx and eae genes. Confirmation of presumptive results was carried out by direct streaking on selective agar and molecular confirmation using the same NEOGEN® assays.
Results demonstrated that the NEOGEN® Molecular Detection Assay 2 provides a reliable and efficient alternative method for STEC detection across a broad range of food matrices. This validation reinforces the suitability of NEOGEN® Molecular Detection Assay 2 as a robust tool to support food safety testing and regulatory compliance for STEC monitoring.
The certificate and the detailed summary report, number 2020LR92, are available on our website.
