Tag: Campden BRI

Three One Plate certificates issued

MicroVal has approved issuing three certificates for One Plate by Neogen Cooperation. Campden BRI has carried out these validation studies of this alternative method based on ISO 16140-2:2016.

The first certificate is One Plate Yeast & Mould. The validation is for the enumeration of yeasts and moulds. This One Plate has been validated against ISO 21527-1:2008 and ISO 21527-2:2008. The scope of validation is a broad range of foods consisting of the following categories:

  1. Heat-processed dairy products
  2. Confectionary, bakery, and eggs (combined category)
  3. Fresh produce and fruit
  4. Ready-to-eat foods (combined category: meat, poultry, and fishery products)
  5. Multicomponent foods or meal components

The second certificate is One Plate Enterobacteriaceae & E.coli. The validation is for the enumeration of Enterobacteriaceae. This second type of One Plate has been validated against ISO 21528-2:2018. Like the first study, the scope of the validation study is a broad range of foods, with categories included:

  1. Milk and dairy products
  2. Fresh produce and fruits
  3. Raw poultry and meats (Combined category raw/ RTC meats and poultry)
  4. Ready-to-eat foods (Combined category RTE/RTRH meats, poultry, and fish)
  5. Multicomponent foods

The last certificate is One Plate Total Viable Count. One Plate for Total Viable Count (OP-TVC) offers a rapid method for enumerating total aerobic mesophiles using traditional culture methodology. It has been validated against ISO 4833-1:2013. Although the scope of the validation study is also a broad range of foods, the categories are:

  1. Heat processed dairy
  2. Raw fishery products
  3. Fresh and heat-processed produce
  4. Multicomponent products
  5. Raw meat and poultry products

On our website, three certificates, 2021LR99, 2022LR108, and 2022LR112, can be found under ‘issued certificates / alternative methods for detection and enumeration‘.

First certificate for Compact Dry CFR

In May 2024, MicroVal approved the issuing of a certificate for Compact Dry CFR, manufactured by Shimadzu Diagnostics Corporation. Compact Dry CFR  features ready-to-use dry media sheets comprising a culture medium and a cold-soluble gelling agent, rehydrated by inoculating 1 ml diluted sample into the center of the self-diffusible medium. This chromogenic plate is designed for enumerating Coliforms. After incubation at 35±1°C for 16-18h, Coliforms develop into blue/blue-green colonies due to chromogens in the medium.

Campden BRI conducted the validation study as the Microval Expert Laboratory, following the ISO 16140-2:2016. The scope of the validation study is two categories: raw milk and dairy products and heat-processed milk and dairy products. For this, the ISO 4832:2006 was used as a reference method. The certificate and the detailed summary report, number 2023LR123, are available on our website.

First MicroVal certificate for commercial sterility detection method

MicroVal has recently issued the certificate for Neogen’s Soleris Commercial Sterility Testing Vials (NF-105) for the determination of commercial sterility in UHT treated milk and dairy alternative plant drinks. These are ready-to-use media vials containing a broth able to grow aerobic, mesophilic microorganisms (bacteria, yeasts and moulds) that are able to grow on an aerobic plate count at 30°C. Results from the validation data showed that the alternative method can be used as a rapid and sensitive testing method for commercial sterility monitoring of UHT treated milk and dairy alternative drinks.

Campden BRI has carried out the validation study of this alternative method, based on ISO 16140-2:2016. The Soleris NF-105 testing vial has been validated against the Codex Alimentarius, Code of Hygienic practice for milk and milk products (CAC/RCP 57-2004), Annex B Microbiocidal control measures Section 2.2 process management for UHT treatment using ISO 4833-1:2013 for the plate count at 30°C. In this validation, the method was used to determine a defined threshold of product contamination of greater than ≤ 10cfu per 0.1ml as defined in the European Directive 92/46 annex C chapter 2 for ultra-high temperature (UHT) milk.

The certificate and the accompanying summary report, number 2021LR100, can be found under ‘issued certificates / alternative methods’.