In June 2022, MicroVal has approved the issuing of the certificate for Bio-Rad’s iQ-Check™ STEC VirX kit for real-time PCR detection of virulence genes in Shiga toxin-producing Escherichia coli. ADRIA Développement has carried out the validation study of this alternative method, based on ISO 16140-2:2016, for the scope of raw meat products (excluding poultry) and raw dairy products.
STEC outbreaks are commonly associated with the consumption of raw meat, particularly beef, but also with dairy products. A sample positive for both stx1/stx2 and eae targets typically requires further testing for the identification of the major E. coli serogroups. The iQ-Check STEC VirX Kit, based on a multiplex real-time PCR system, allows the detection of the stx1/stx2 and eae virulence genes within a few hours after microbiological enrichment.
The certificate and the accompanying validation report, number 2021LR96, can be found under ‘issued certificates / alternative methods’.